What is CpG ODN
CpG ODNs are single-stranded synthetic oligodeoxyribonucleotides containing unmethylated CpG motifs derived from bacterial DNA. These are an artificial ligand molecule for TLR9 and a potent Th1-type adjuvant that induces inflammatory cytokines, B cell proliferation, dendritic cell maturation, and NK cell activation.
These have been suggested to be effective not only as vaccines but also as single agents as antiallergic and anti-tumor drugs.
Classification of CpG ODN
Three major classes of stimulatory CpG ODNs are Class A (Type D), Class B (Type K) and Class C.
- Class A CpG ODNs are characterized by a PO central CpG-containing palindromic motif and a PS-modified 3’ poly-G string. They induce high IFN-α production from pDCs but are weak stimulators of TLR9-dependent NF-κB signaling and pro-inflammatory cytokine (e.g. IL-6) production.
- Class B CpG ODNs contain a full PS backbone with one or more CpG dinucleotides. They strongly activate B cells and TLR9-dependent NF-κB signaling but weakly stimulate IFN-α secretion.
- Class C CpG ODNs combine features of both classes A and B. They contain a complete PS backbone and a CpG-containing palindromic motif. C-Class CpG ODNs induce strong IFN-α production from pDC as well as B cell stimulation.
Class A CpG ODNs
Class B CpG ODNs
For other types, please contact us.